The introduction of Sulforhodamine G

Sulforhodamine G is used as a polar tracer as sulforhodamine B. Compared to rhodamine B it is more fluorescent. Fig 8  Comparison of uranine and sulforhodamine G. a BTCs measured at inspection well 20DX inside the tunnel: the uranine BTC shows 20 times higher maximum concentrations and a shorter tail than the sulforhodamine G curve, although uranine was injected further upstream in Veccione Stream, suggesting that uranine behaves more conservatively in fractured turbidites (injection and sampling site are shown in Fig. ). b) Uranine and sulforhodamine G BTCs measured by means of a continuous fluorometer at the total drainage of the northern sector of the tunnel.

The uranine recovery is about 100 times higher than the sulforhodamine G recovery, further confirming the more conservative behaviour of uranine.In our initial experiments, we microinjected a range of fluorescent probes (fluorescein, Lucifer yellow CH [LYCH], sulforhodamine G) into the basal trichome cell to determine symplasmic continuity between the basal trichome cell and the underlying epidermis (Fig. 1A ). As all dyes gave the same result, we present here data for the most membrane impermeant of these, LYCH. Given the large numbers of PD at the e/t interface (Faulkner et al., 2008), we were surprised that in 35 of 39 injections, LYCH failed to move across the e/t boundary and enter the epidermis (Fig. 1A).

However, in each injection, LYCH moved apically into other trichome cells (Fig. 1A). In all cases, cytoplasmic streaming resumed immediately after dye injection. When dye was observed to have crossed the e/t barrier, only extremely weak fluorescence was observed in the underlying epidermal cell. In contrast, if we injected LYCH into the supporting epidermal cell, dye moved apically across the e/t boundary into the trichome (Fig. 1B). With time (1–3 h), all dyes were sequestered slowly into the central vacuoles of the trichome cells, sulforhodamine G showing the highest rate of vacuolar sequestration .
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