All strains grew on BAM agar containing 2 % (w/v) NaCl. Phenylalanine and tyrosine were not hydrolysed. Acid was produced from L-arabinose, ribose, D-xylose, D-galactose, D-glucose, D-fructose, D-mannose and trehalose, but not from ribitol, sorbitol, N-acetylglucosamine, L-arabitol, gluconate, 2-ketogluconate and 5-ketogluconate. All strains produced spores within several days of growth (BAM agar), with the exception of strain S-TABT, which produced only small amounts of spores after 10 days. In addition, many vegetative cells of strain S-TABT on YSG agar died within 7 days and therefore repeated subculturing was difficult using the most popular YSG medium for cultivation of Alicyclobacillus. The diameter of swollen sporangia from strain 5-A239-2O-AT was more than three times that of vegetative cells.
6-TAMRA, 6-Carboxytetramethylrhodamine is a single isomer free acid which can be used for labeling free amines on proteins. TAMRA excites at 560 nm and emits at 583 nm.This single isomer amine-reactive red fluorescent derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.
The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.
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